Journal: Journal of Clinical Virology plus

Article Title: Detection of SARS-CoV-2 VOC-Omicron using commercial sample-to-answer real-time RT-PCR platforms and melting curve-based SNP assays

doi: 10.1016/j.jcvp.2022.100091

Figure Lengend Snippet: Sensitivity and specificity of TIB MOLBIOL VirSNiP SARS-CoV-2 Spike SNP assays with reference to whole genome sequencing.

Article Snippet: TIB MOLBIOL VirSNiP SARS-CoV-2 Spike S371L/S373P assay , S371L/S373P , , S371S/S373S , Ab Tm , No Tm , Omicron S371L/S373P , , Others S371S/S373S , .

Techniques: Sequencing

Journal: bioRxiv

Article Title: Hybrid immunity shifts the Fc-effector quality of SARS-CoV-2 mRNA vaccine-induced immunity

doi: 10.1101/2022.06.10.495727

Figure Lengend Snippet: Isotype titers and FcR binding for previously infected individuals after dose 1 are comparable to levels in naïve individuals after dose 2 A. The heatmap shows Z-scored SARS-CoV-2 specific antibody features for individuals post-prime and post-boost. Each row is an individual sample, and each column is a measured feature. Features are clustered hierarchically, and individuals are also clustered within each group (infected / not infected and Pfizer-BioNTech / Moderna). Z-scores are calculated across all samples and truncated between -4 and 4. B. Violin plots show individual subclasses specific for S1, RBD, S2, and NTD for naïve infected and previously infected individuals after 1 st and 2 nd vaccine doses. Significance was determined by a non-parametric Kruskal-Wallis test followed by Dunn’s multiple comparisons test between pairs of groups. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. C. Violin plots show low affinity FcR binding by S1, RBD, S2, and NTD-specific antibodies for naïve and previously infected individuals after 1 st and 2 nd vaccine doses. Significance was determined by a non-parametric Kruskal-Wallis test followed by Dunn’s multiple comparisons test between pairs of groups. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Antigens used for Luminex based assays: SARS-CoV-2 D614G WT S (provided by Erica Saphire, La Jolla Institute for Immunology), SARS-CoV-2 S1 (Sino Biological), SARS-CoV-2 S2 (Sino Biological) and SARS-CoV-2 RBD (kindly provided by Aaron Schmidt, Ragon Institute), and SARS-CoV-2 NTD (Sino Biological).

Techniques: Binding Assay, Infection

Journal: iScience

Article Title: ACE2 interaction with cytoplasmic PDZ protein enhances SARS-CoV-2 invasion

doi: 10.1016/j.isci.2021.102770

Figure Lengend Snippet:

Article Snippet: Spike (SARS-CoV-2) Pseudotyped Lentivirus (Luciferase Reporter) , BPS Bioscience , Cat#79942-1.

Techniques: Recombinant, Bicinchoninic Acid Protein Assay, In Situ, Proximity Ligation Assay, Luciferase, Software

Journal: Cell

Article Title: Immune imprinting, breadth of variant recognition, and germinal center response in human SARS-CoV-2 infection and vaccination

doi: 10.1016/j.cell.2022.01.018

Figure Lengend Snippet:

Article Snippet: S-PLEX SARS-CoV-2 Spike Kit , Meso Scale Discovery , Cat#K150ADJS.

Techniques: Clinical Proteomics, Vaccines, Infection, Control, Recombinant, RNAscope, Positive Control, Polymer, Titration, Electrochemiluminescence, Virus, Neutralization, Software